ct26 cl25 cells  (ATCC)

Journal: Nature Communications

Article Title: Bioinspired ruthenium-manganese-oxygen complex for biocatalytic and radiosensitization therapies to eradicate primary and metastatic tumors

doi: 10.1038/s41467-025-62999-x

Figure Lengend Snippet: a A schematic illustration of the in vitro antitumor activities of MnBTC-Ru. b The representative fluorescence images and c quantitative flow cytometry show the ROS generation in CT26 cells in different groups ( n = 3 independent replicates; scale bar = 100 μm). d The intracellular ATP of CT26 in different groups ( n = 3 independent replicates). e LPO product MDA detection of CT26 cells after different treatments ( n = 3 independent replicates). f The expression of HIF-1α in CT26 subjected to different treatments (scale bar = 50 μm). g The Annexin V/PI analysis of CT26 cells in different groups ( n = 3 independent replicates). The graph showed the percentage of apoptotic cells (early apoptotic, late apoptotic) in different groups ( n = 3 independent replicates). h Live/Dead analysis of CT26 cells after different treatments, Green: live cells; Red: dead cells (scale bar = 100 μm). i Transwell migration assay is used to evaluate cell migration, and the graphs show the numbers of migrated cells in different groups ( n = 3 independent replicates). j A schematic diagram of ICD of tumor cells releasing DAMPs and interacting with dendritic cells. k The ATP release of CT26 in different groups ( n = 3 independent replicates). l The representative fluorescence images show the expression of CRT in CT26 after different treatments (scale bar = 10 μm). Experiments were repeated independently ( b, c, f, h, l ) three times with similar results. DAPI indicates 4′,6-diamidino-2-phenylindole, PI indicates propidium iodide, FITC indicates fluorescein isothiocyanate, Calcein-AM indicates calcein acetoxymethyl ester. Results are presented as means ± SD. Statistical significance was assessed using the Student’s t-test for two-group comparisons and one-way ANOVA for multiple-group comparisons, followed by Tukey’s two-tailed post-hoc test for pairwise analysis, all tests were two-sided. Source data are provided as a file.

Article Snippet: The CT26 cell line (Cat CRL-2639) was sourced from the American Type Culture Collection (ATCC) and verified for authenticity through short tandem repeat analysis.

Techniques: In Vitro, Fluorescence, Flow Cytometry, Expressing, Transwell Migration Assay, Migration, Two Tailed Test

Journal: Nature Communications

Article Title: Bioinspired ruthenium-manganese-oxygen complex for biocatalytic and radiosensitization therapies to eradicate primary and metastatic tumors

doi: 10.1038/s41467-025-62999-x

Figure Lengend Snippet: a Schematic illustration of RT+MnBTC-Ru treatment. b Average tumor growth curves, c individual tumor growth kinetics, d tumor weight, and e body weight of CT26 tumor-bearing mice after different treatments ( n = 5 biologically independent mice per group). f Representative H&E and fluorescent staining images (TUNEL assay, γ-H2AX and Ki67 staining) from tumor tissue section (scale bar = 100 μm). g Representative H&E-stained images from major organ tissue section (scale bar = 100 μm). h Results of blood chemistry parameters from mice after different treatments ( n = 3 independent replicates). Blood chemistry parameters include white blood cell (WBC), red blood cell (RBC), platelet count (PLT), hemoglobin (HGB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), albumin (ALB), and Creatine Kinase (CK). i Representative flow cytometric analysis and relative quantification of CD3 + T cells, CD8 + T cells, and CD8 + CD69 + T cells ( n = 3 independent replicates). In ( b – i ), the Control group indicates saline. Experiments were repeated independently ( f, g ) three times with similar results. Ki67 indicates Marker of Proliferation Ki67, γ-H2AX indicates phosphorylated histone H2A.X at Ser139, SSC-A indicates Side Scatter Area, BUV395 indicates Brilliant Ultraviolet 395, BUV661 indicates Brilliant Ultraviolet 661, BV771 indicates Brilliant Violet 771, PE-Cy7 indicates Phycoerythrin–Cyanine 7. Results are presented as means ± SD. Statistical significance was determined using the one-way ANOVA for multiple-group comparisons, followed by Tukey’s two-tailed post-hoc test for pairwise analysis, all tests were two-sided. Source data are provided as a file.

Article Snippet: The CT26 cell line (Cat CRL-2639) was sourced from the American Type Culture Collection (ATCC) and verified for authenticity through short tandem repeat analysis.

Techniques: Staining, TUNEL Assay, Quantitative Proteomics, Control, Saline, Marker, Two Tailed Test

Journal: Nature Communications

Article Title: Bioinspired ruthenium-manganese-oxygen complex for biocatalytic and radiosensitization therapies to eradicate primary and metastatic tumors

doi: 10.1038/s41467-025-62999-x

Figure Lengend Snippet: a Schematic illustration of RT+MnBTC-Ru with anti-PD-1 treatment (i.p., intraperitoneal). b Individual tumor growth kinetics of distant tumor, average tumor growth curves of c primary tumor and d distant tumor, e tumor weight, and f body weight of bilateral CT26 tumor-bearing mice after different treatments ( n = 5 biologically independent mice per group). g Representative immunofluorescence images from lymph node slices stained with DAPI (blue) and CD8 (red) antibodies (scale bar = 400 μm). h Schematic illustration of the experiment design to assess the antitumor memory responses triggered by RT+MnBTC-Ru+anti-PD-1 combination therapy. i Average tumor growth curves and j individual tumor growth kinetics of the treated mice ( n = 5 biologically independent mice per group). k – o Representative flow cytometric analysis of Control and RT+MnBTC-Ru+anti-PD-1 group, with corresponding quantification of CD45 + , CD3 + T, CD8 + T and CD4 + T cells, and subsets Tcms (CD62L + CD44 + ) and Tems (CD62L - CD44 + ) from CD8 + and CD4 + T cells in the spleen ( n = 3 independent replicates). In ( b - g ) and ( i - o ), the Control group indicates saline. Experiments were repeated independently ( g ) three times with similar results. L/D indicates Live/Dead, FVS440UV indicates Fixable Viability Stain 440UV, APC-Cy7 indicates Allophycocyanin–Cyanine 7, PerCP-Cy5.5 indicates Peridinin–Chlorophyll–Protein–Cyanine 5.5. Results are presented as means ± SD. Statistical significance was determined using the Student’s t-test for two-group comparisons, and one-way ANOVA for multiple-group comparisons, followed by Tukey’s two-tailed post-hoc test for pairwise analysis, all tests were two-sided. Source data are provided as a Source Data file.

Article Snippet: The CT26 cell line (Cat CRL-2639) was sourced from the American Type Culture Collection (ATCC) and verified for authenticity through short tandem repeat analysis.

Techniques: Immunofluorescence, Staining, Control, Saline, Two Tailed Test